| 2024 | 22.01 | Pan American | STEPHENS Lauren | DOS SANTOS RODRIGUES Wellyda Regisleyne | SOTO CAMPOS Catalina Anais | Road race |
| 2024 | 22.01 | Pan American | NEBEN Amber Leone | STEPHENS Lauren | VILLALON SANCHEZ Aranza Valentina | Time trial |
| 2023 | 22.01 | Pan American | NEBEN Amber Leone | VILLALON SANCHEZ Aranza Valentina | JACKSON Alison | Time trial |
| 2023 | 22.01 | Pan American | SCHNEIDER Skylar | JACKSON Alison | SOTO CAMPOS Catalina Anais | Road race |
| 2022 | 22.01 | Pan American | CHACON GARCIA Lilibeth De Carmen | HERNANDEZ GOMEZ Lina Marcela | LEONARDI Antonella Lila | Time trial |
| 2022 | 22.01 | Pan American | SIERRA CANADILLA Arlenis | SOTO CAMPOS Catalina Anais | GRANIZAL Fabiana | Road race |
| 2021 | 22.01 | Pan American | MEJIAS GARCIA Marlies | HERNANDEZ GOMEZ Lina Marcela | VILLALON SANCHEZ Aranza Valentina | Time trial |
| 2021 | 22.01 | Pan American | HERNANDEZ GOMEZ Lina Marcela | MUNOZ GRANDON Paola | CAMPBELL Teniel | Road race |
| 2019 | 22.01 | Pan American | THOMAS Leah | NEBEN Amber Leone | Constanza Paredes | Time trial |
| 2019 | 22.01 | Pan American | GUTIERREZ ARZALUZ Ariadna | Denisse Ahumada | CAMPBELL Teniel | Road race |
| 2018 | 22.01 | Pan American | NEBEN Amber Leone | STEPHENS Lauren | Ana Sanabria | Time trial |
| 2018 | 22.01 | Pan American | SIERRA CANADILLA Arlenis | GARCIA OCASIO Iraida | MEJIAS GARCIA Marlies | Road race |
| 2017 | 22.01 | Pan American | DYGERT Chloe | WILES Tayler | MEJIAS GARCIA Marlies | Time trial |
| 2017 | 22.01 | Pan American | MUNOZ GRANDON Paola | Wellynda dos Santos | SCHNEIDER Skylar | Road race |
| 2016 | 22.01 | Pan American | GARCIA OCASIO Iraida | SIERRA CANADILLA Arlenis | DE OLIVEIRA PAPARELLA Flavia Maria | Road race |
| 2016 | 22.01 | Pan American | Sérika Gulumá | Ana Sanabria | DREXEL CLOUTHIER Ingrid | Time trial |
| 2015 | 22.01 | Pan American | SMALL Carmen | WHITTEN Tara | FERNANDES SILVA Clemilda | Time trial |
| 2015 | 22.01 | Pan American | MEJIAS GARCIA Marlies | Coryn Rivera | GONZALEZ Yumari | Road race |
| 2014 | 22.01 | Pan American | SIERRA CANADILLA Arlenis | GUARNIER Megan | Laura Lozano | Road race |
| 2014 | 22.01 | Pan American | STEVENS Evelyn | Sérika Gulumá | GUARNIER Megan | Time trial |
| 2013 | 22.01 | Pan American | SIERRA CANADILLA Arlenis | MEJIAS GARCIA Marlies | Angie González | Road race |
| 2013 | 22.01 | Pan American | DREXEL CLOUTHIER Ingrid | SMALL Carmen | Sérika Gulumá | Time trial |
| 2012 | 22.01 | Pan American | GONZALEZ Yumari | Leah Kirchmann | FERNANDES SILVA
Janildes | Road race |
| 2012 | 22.01 | Pan American | NEBEN Amber Leone | Rhae-Christie Shaw | FERNANDES SILVA Clemilda | Time trial |
| 2011 | 22.01 | Pan American | HUGHES Clara | Evelyn García | Theresa Cliff-Ryan | Road race |
| 2011 | 22.01 | Pan American | HUGHES Clara | STEVENS Evelyn | NEBEN Amber Leone | Time trial |
| 2010 | 22.01 | Pan American | Shelley Evans Olds | NUMAINVILLE Joëlle | Dalila Rodríguez | Road race |
| 2010 | 22.01 | Pan American | MADRINAN Ana Paola | NEBEN Amber Leone | Shelley Evans | Time trial |
| 2009 | 22.01 | Pan American | NUMAINVILLE Joëlle | MUNOZ GRANDON Paola | Verónica Leal | Road race |
| 2009 | 22.01 | Pan American | GRASSI HERRERA Alessandra Giuseppina | WHITTEN Tara | Valeria Müller | Time trial |
| 2008 | 22.01 | Pan American | GONZALEZ Yumari | Karelia Machado | BELEM Guerrero | Road race |
| 2008 | 22.01 | Pan American | MADRINAN Ana Paola | GRASSI HERRERA Alessandra Giuseppina | Kori Kelley Seehafer | Time trial |
| 2007 | 22.01 | Pan American | MAYOLO-PIC Tina | Yumari González | Gina Grain | Road race |
| 2007 | 22.01 | Pan American | POWERS Alison | GRASSI HERRERA Alessandra Giuseppina | BAUSCH Dotsie | Time trial |
| 2006 | 22.01 | Pan American | NEBEN Amber Leone | Erinne Willock | Kori Kelley Seehafer | Time trial |
| 2006 | 22.01 | Pan American | GONZALEZ Yumari | Kori Kelley Seehafer | FERNANDES SILVA Clemilda | Road race |
| 2005 | 22.01 | Pan American | ARMSTRONG Kristin | THORNBURN Christine | MADRINAN Ana Paola | Time trial |
| 2005 | 22.01 | Pan American | MAYOLO-PIC Tina | FERNANDEZ Yeilien | GONZALEZ Yumari | Road race |
| 2004 | 22.01 | Pan American | GONZALEZ Yoanka | FERNANDEZ Yeilien | Danielys García | Road race |
| 2004 | 22.01 | Pan American | MADRINAN Ana Paola | Marie Rosado | GONZALEZ Yoanka | Time trial |
| 2002 | 22.01 | Pan American | Sonia López | MADRINAN Ana Paola | DELGADILLO RUIZ Flor Marina | Time trial |
| 2002 | 22.01 | Pan American | BELEM Guerrero | DELGADILLO RUIZ Flor Marina | FERNANDES SILVA
Janildes | Road race |
| 2001 | 22.01 | Pan American | Luz Delgadillo | BELEM Guerrero | Martha López | Road race |
| 2001 | 22.01 | Pan American | María Luisa Calle | DELGADILLO RUIZ Flor Marina | Julier Rodríguez | Time trial |
| 1996 | 22.01 | Pan American | Nicole Reinhart | Madelin Jorge | Anrrosy Paruta | Road race |